Simultaneous HPLC-DAD quantification of vitamins A and E content in raw, pasteurized, and UHT cow’s milk and their changes during storage

نویسندگان

  • Nattaporn Chotyakul
  • Miriam Pateiro-Moure
  • Jorge Alexandre Saraiva
  • J. Antonio Torres
  • Concepción Pérez-Lamela
چکیده

An improved extraction and HPLC method for the simultaneous extraction and quantitation of retinol, α-tocopherol, α-tocotrienol and β-carotene was developed to analyze commercial whole/semi-skim/skim samples of raw/pasteurized/UHT milk in transparent plastic/glass bottles and Tetra Brik containers. The sample preparation method required prior saponification at 40oC for 15 min followed by n-hexane extraction. An isocratic acetonitrile:methanol (65:35 v/v) mobile phase and UV detection were chosen for HPLC quantification. The liposoluble vitamin content in raw, pasteurized conventional/organic, and UHT milk ranged 0.055-5.540 (retinol), 0.1351.410 (α-tocopherol), and 0.040-0.850 mg/L (β-carotene). No significant differences (p>0.05) were observed on losses of retinol, α-tocopherol, and β-carotene content in UHT whole milk after 5 days at 4oC in the dark. After 14 days at 4oC in the dark the contents of retinol, α-tocopherol, and β-carotene remained higher in milk with higher fat content and were higher in unopened containers. In UHT whole milk samples containing 0.02% NaN3, retinol (33%) and α-tocopherol (11%) but not β-carotene (2%) decreased significantly (p<0.05).

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تاریخ انتشار 2014